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nanofil needles-nf36bv-2  (World Precision Instruments)


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    Structured Review

    World Precision Instruments nanofil needles-nf36bv-2
    Nanofil Needles Nf36bv 2, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 95/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/needle/NanoFil+Needles-NF36BV-2/custom%40nf36bv-2%4042589157
    Average 95 stars, based on 208 article reviews
    nanofil needles-nf36bv-2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: A gatekeeper sympathetic control of lacrimal tear secretion and dry eye onset through the NA-Adra1a-Ucp2 pathway
    Article Snippet: .. AAV solution at 2 μL was injected using a 36 G beveled needle (World Precision Instruments, #NF36BV-2) mounted on a 10 μL microsyringe (World Precision Instruments, #NanoFil 300,329). .. Each entire lacrimal gland per mouse was fixed in 10% formalin and paraffin-embedded, sections (4 μm) were incubated with rabbit anti-CD45 (1:50, Abcam; #ab10558) overnight at 4 °C and subsequently incubated with secondary antibodies and visualized using DAB detection kit (MXB, #DAB-0031) according to the manufacturer’s instructions.

    Injection:

    Article Title: A gatekeeper sympathetic control of lacrimal tear secretion and dry eye onset through the NA-Adra1a-Ucp2 pathway
    Article Snippet: .. AAV solution at 2 μL was injected using a 36 G beveled needle (World Precision Instruments, #NF36BV-2) mounted on a 10 μL microsyringe (World Precision Instruments, #NanoFil 300,329). .. Each entire lacrimal gland per mouse was fixed in 10% formalin and paraffin-embedded, sections (4 μm) were incubated with rabbit anti-CD45 (1:50, Abcam; #ab10558) overnight at 4 °C and subsequently incubated with secondary antibodies and visualized using DAB detection kit (MXB, #DAB-0031) according to the manufacturer’s instructions.

    Article Title: A homeostatic gut-to-brain insulin antagonist restrains neuronally stimulated fat loss.
    Article Snippet: 2.5μL GFP repair template (1μM, IDT), 0.5μL dpy-10 repair template (10μM, IDT), and 1.84μL nuclease-free water were added to the mix and incubated at 25 °C for 60min. .. The final injection mix was loaded using a pulled capillary needle (1B100F-4, World Precision Instruments) and injected into the germline of N2 young adults. ..

    Article Title: A homeostatic gut-to-brain insulin antagonist restrains neuronally stimulated fat loss
    Article Snippet: Steps for CRISPR-Cas9 gene editing are briefly described as follows: 0.88 μL tracrRNA (200 μM, IDT), 0.82 μL ins-7 guide RNAs (100 μM, IDT), and 0.12 μL dpy-10 guide RNA (100 μM, IDT) were mixed and incubated at 95 °C for 5 min. 2.52 μL Cas9 protein (IDT, Catalog# 1081058) was added to the mix and incubated at 25 °C for 5 min. 2.5 μL GFP repair template (1 μM, IDT), 0.5 μL dpy-10 repair template (10 μM, IDT), and 1.84 μL nuclease-free water were added to the mix and incubated at 25 °C for 60 min. .. The final injection mix was loaded using a pulled capillary needle (1B100F-4, World Precision Instruments) and injected into the germline of N2 young adults. ..

    Article Title: Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins
    Article Snippet: .. Virus suspensions were slowly injected (100 nl min −1 ) using a 34 G beveled needle (Nanofil syringe, World Precision Instruments). ..

    Article Title: Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins
    Article Snippet: .. Virus suspensions were slowly injected (100 nl min−1) using a 34 G beveled needle (Nanofil syringe, World Precision Instruments). ..

    Microinjection:

    Article Title: Multifunctional Neural Probes Enable Bidirectional Electrical, Optical, and Chemical Recording and Stimulation In Vivo.
    Article Snippet: The device was then cemented to the skull using 3 layers of C&B-Metabond adhesive acrylic (Parkell) followed by dental cement (Jet Set-4) to cover the base of the device and the skull screws. .. For the single NAc-targeting fiber (Figure S14, Supporting Information), a microinjection apparatus (NanoFil Syringe and UMP-3 Syringe Pump, World Precision Instruments) was used to load 1.5 μl of a 1:1 mixture of AAV9-hSynGRAB_rDA1 m and AAV5-EF1α-DIO-hChR2(H134R)-mCherry through a 35 G needle (NF35BV-2, World Precision Instruments). ..

    Virus:

    Article Title: Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins
    Article Snippet: .. Virus suspensions were slowly injected (100 nl min −1 ) using a 34 G beveled needle (Nanofil syringe, World Precision Instruments). ..

    Article Title: Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins
    Article Snippet: .. Virus suspensions were slowly injected (100 nl min−1) using a 34 G beveled needle (Nanofil syringe, World Precision Instruments). ..



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